Countercurrent enzymatic saccharification of pretreated corn stover. Part 2: Lime + shock pretreated corn stover and commercial approach Academic Article

abstract

• 2017 Elsevier Ltd Corn stover was pretreated with submerged lime plus shock, a mechanical treatment that subjects biomass to a detonation wave. In a series of bottles, pretreated corn stover was countercurrently saccharified with enzyme loadings of CTec3 (1mg protein/g dry biomass), CTec3 (1mg protein/g dry biomass)+HTec3 (1mg protein/g dry biomass), and CTec3 (2mg protein/g dry biomass)+HTec3 (2mg protein/g dry biomass). To reach a given glucan conversion at low enzyme loadings, countercurrent saccharification reduced enzyme loadings by 1.9 times compared with batch saccharification but was not helpful at high enzyme loadings. To preserve the sugars during saccharification, volatile anti-microbials (chloroform and essential oils from thyme and oregano) prevent the growth of contaminating microorganisms. A simple packed column is proposed to perform commercial-scale countercurrent saccharification.

authors

• Holtzapple, Mark

published proceedings

• BIOMASS & BIOENERGY

author list (cited authors)

• Liang, C., Lonkar, S., Darvekar, P., Bond, A., Zentay, A. N., Holtzapple, M. T., & Karim, M. N.

citation count

• 1

complete list of authors

• Liang, Chao||Lonkar, Sagar||Darvekar, Pratik||Bond, Austin||Zentay, Agustin N||Holtzapple, Mark T||Karim, M Nazmul

publication date

• March 2017

publisher

• Elsevier  Publisher

published in

• Biomass and Bioenergy  Journal

keywords

• Countercurrent
• Enzymatic Hydrolysis
• Lignocellulose
• Pretreated Corn Stover
• Saccharification

Digital Object Identifier (DOI)

• 10.1016/j.biombioe.2017.01.008

start page

• 124

end page

• 134

volume

• 98

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.biombioe.2017.01.008