In vitro interaction between oviduct epithelial and equine sperm.
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Coculture of stallion sperm with monolayers of equine oviductal epithelial cells (OEC) was evaluated. Monolayers were obtained from frozen-thawed OEC. Live sperm attached to the OEC in vitro, whereas sperm killed by heat treatment or glutaraldehyde fixation did not. Sperm attached to OEC showed flagellar motion for 4 d in vitro, during which time they gradually became released. Scanning electron-micrographs showed an intimate association between the sperm and OEC. Incubation of sperm for 4 h with either control, heparinized or OEC-conditioned medium (Tyrode's albumin lactate phosphate) resulted in more incapacitated sperm, as determined by chlortetracycline staining patterns. The OEC-conditioned medium caused similar capacitation-like changes to those seen with heparin. Sperm viability as determined by Hoechst 33258 staining was not significantly affected by media type.
author list (cited authors)
Ellington, J. E., Ignotz, G. G., Varner, D. D., Marcucio, R. S., Mathison, P., & Ball, B. A
complete list of authors
Ellington, JE||Ignotz, GG||Varner, DD||Marcucio, RS||Mathison, P||Ball, BA