In vitro interaction between oviduct epithelial and equine sperm. Academic Article uri icon

abstract

  • Coculture of stallion sperm with monolayers of equine oviductal epithelial cells (OEC) was evaluated. Monolayers were obtained from frozen-thawed OEC. Live sperm attached to the OEC in vitro, whereas sperm killed by heat treatment or glutaraldehyde fixation did not. Sperm attached to OEC showed flagellar motion for 4 d in vitro, during which time they gradually became released. Scanning electron-micrographs showed an intimate association between the sperm and OEC. Incubation of sperm for 4 h with either control, heparinized or OEC-conditioned medium (Tyrode's albumin lactate phosphate) resulted in more incapacitated sperm, as determined by chlortetracycline staining patterns. The OEC-conditioned medium caused similar capacitation-like changes to those seen with heparin. Sperm viability as determined by Hoechst 33258 staining was not significantly affected by media type.

published proceedings

  • Arch Androl

author list (cited authors)

  • Ellington, J. E., Ignotz, G. G., Varner, D. D., Marcucio, R. S., Mathison, P., & Ball, B. A

citation count

  • 42

complete list of authors

  • Ellington, JE||Ignotz, GG||Varner, DD||Marcucio, RS||Mathison, P||Ball, BA

publication date

  • September 1993