In vitro interaction between oviduct epithelial and equine sperm. Academic Article

abstract

• Coculture of stallion sperm with monolayers of equine oviductal epithelial cells (OEC) was evaluated. Monolayers were obtained from frozen-thawed OEC. Live sperm attached to the OEC in vitro, whereas sperm killed by heat treatment or glutaraldehyde fixation did not. Sperm attached to OEC showed flagellar motion for 4 d in vitro, during which time they gradually became released. Scanning electron-micrographs showed an intimate association between the sperm and OEC. Incubation of sperm for 4 h with either control, heparinized or OEC-conditioned medium (Tyrode's albumin lactate phosphate) resulted in more incapacitated sperm, as determined by chlortetracycline staining patterns. The OEC-conditioned medium caused similar capacitation-like changes to those seen with heparin. Sperm viability as determined by Hoechst 33258 staining was not significantly affected by media type.

authors

• Varner, Dickson

published proceedings

• Arch Androl

author list (cited authors)

• Ellington, J. E., Ignotz, G. G., Varner, D. D., Marcucio, R. S., Mathison, P., & Ball, B. A.

citation count

• 43

complete list of authors

• Ellington, JE||Ignotz, GG||Varner, DD||Marcucio, RS||Mathison, P||Ball, BA

publication date

• January 1993

publisher

• Taylor & Francis  Publisher

published in

• Systems Biology in Reproductive Medicine  Journal

keywords

• Animals
• Cells, Cultured
• Epithelium
• Fallopian Tubes
• Female
• Horses
• Male
• Sperm Capacitation
• Spermatozoa

PubMed Central ID

• 8215695

Digital Object Identifier (DOI)

• 10.3109/01485019308988384

start page

• 79

end page

• 86

volume

• 31

issue

• 2

URL

• http%3A%2F%2Fdx.doi.org%2F10.3109%2F01485019308988384