Development of RAPD markers linked to the male-sterile ms-3 gene in melon
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abstract
The male-sterile gene (ms-3) identified in muskmelon (Cucumis melo L.) has been introgressed into fertile melon cultivars/breeding lines for use as F1 hybrid parents. Molecular markers linked to the ms-3 gene would be useful in transferring the male-sterile gene into elite cultivars/lines using backcrossing. However, markers linked to the ms-3 gene in melon have not been reported. Our objective was to identify random amplified polymorphic DNA (RAPD) markers linked to the malesterile ms-3 gene using bulked segregant analysis in an F2 population from the melon cross of ms-3 (male-sterile) x 'Dulce' (male-fertile). The parents, 141 F2 plants, and 47 F3 families (18 plants per F3) were planted in two separate greenhouse experiments. A single recessive gene controlling the male-sterile trait was found in the F2 and confirmed in the F3. A total of 680 primers was screened between two different bulks prepared from homozygous male-sterile and fertile F2 plants. Two RAPD markers OAM08.650 and OAN05.800 that displayed an amplified DNA fragment in the malesterile bulk were detected to be linked to the ms-3 gene. Marker OAM08.650 was the most closely linked to the ms-3 gene at a distance of 2.1 cM. This marker was also present in each of 18 heterozygous fertile melon maintainers carrying the ms-3 gene. Marker OAM08.650 closely linked to the ms-3 gene identified here could be used for rapid backcrossing of the male-sterile gene into elite melon cultivars/lines.
