Hyper-Rayleigh scattering as a method for probing collagen transformations in solutions
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Hyper-Rayleigh scattering is used to measure the nonlinear optical properties of collagen type I in acetic acid solution. We find that the first hyperpolarizability unexpectedly drops with the increase of the concentration of collagen in solution. Circular dichroism absorption spectroscopy and UV absorption measurements indicate that the helical conformation remains stable, and the changes of nonlinear optical properties of collagen molecules are, most probably, due to the formation of some supramolelcular structures of collagen in solution.
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Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III