Quantum Control of Population Transfer in Green Fluorescent Protein by Using Chirped Femtosecond Pulses Academic Article uri icon

abstract

  • We demonstrate that the methods of quantum control can be applied successfully to very large molecules in room temperature liquid solution. Chirped femtosecond pulses are used to excite a green fluorescent protein mutant in both buffered aqueous solution and solid acrylamide gel. At high energy densities, the fluorescence shows a strong chirp dependence, with positively chirped pulses transferring almost 50% more population to the excited state than negatively Chirped pulses. By measuring the photobleaching rate in the gel as a function of pulse chirp, we find that the data are consistent with the bleaching of the protein being due to a thermal mechanism rather than to an excited-state photoreaction.

altmetric score

  • 3

author list (cited authors)

  • Bardeen, C. J., Yakovlev, V. V., Squier, J. A., & Wilson, K. R.

citation count

  • 105

complete list of authors

  • Bardeen, Christopher J||Yakovlev, Vladislav V||Squier, Jeff A||Wilson, Kent R

publication date

  • December 1998