Quantum control of population transfer in green fluorescent protein by using chirped femtosecond pulses Academic Article uri icon


  • We demonstrate that the methods of quantum control can be applied successfully to very large molecules in room temperature liquid solution. Chirped femtosecond pulses are used to excite a green fluorescent protein mutant in both buffered aqueous solution and solid acrylamide gel. At high energy densities, the fluorescence shows a strong chirp dependence, with positively chirped pulses transferring almost 50% more population to the excited state than negatively Chirped pulses. By measuring the photobleaching rate in the gel as a function of pulse chirp, we find that the data are consistent with the bleaching of the protein being due to a thermal mechanism rather than to an excited-state photoreaction.

published proceedings


altmetric score

  • 3

author list (cited authors)

  • Bardeen, C. J., Yakovlev, V. V., Squier, J. A., & Wilson, K. R.

citation count

  • 104

complete list of authors

  • Bardeen, CJ||Yakovlev, VV||Squier, JA||Wilson, KR

publication date

  • December 1998