Subcellular imaging of mechanical and chemical properties using Brillouin and Raman microspectroscopies
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2015 SPIE. Brillouin and Raman microspectroscopies are powerful techniques for noninvasive optical imaging. In particular, Brillouin microscopy is a unique technique, which allows assessing samples mechanical properties with optical level spatial resolution. Recent advances in background-free Brillouin microspectroscopy make it possible to image turbid samples without substantial degradation of the data quality. In this report, we applied Brillouin spectroscopy to imaging individual cells. These viscoelastic measurements were supplemented by chemically specific measurements using confocal Raman microspectroscopy.
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Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII