Watching embryonic development in a new light: Elasticity specific imaging with dual Brillouin/Raman microspectroscopy Conference Paper

abstract

• 2016 SPIE. Mechanical properties of tissues play an important role in biological development. However, the current elasticity-specific imaging techniques are either destructive/invasive, or have a limited spatial and/or temporal resolution. Recently, we introduced Brillouin microscopy imaging as a local non-invasive probe of microscopic viscoelasticity in cells and tissues. In this study, by taking advantage of Brillouin spectroscopy, we imaged the viscoelasticity properties of different compartments of living zebrafish embryos, including yolk-sac, skin, spine and heart. Brillouin and Raman spectra were collected simultaneously at each location using a recently developed Brillouin/Raman microscope.

name of conference

• Optical Methods in Developmental Biology IV

authors

• Yakovlev, Vladislav

published proceedings

• OPTICAL METHODS IN DEVELOPMENTAL BIOLOGY IV

altmetric score

• 3

author list (cited authors)

• Meng, Z., Hanson, J. A., & Yakovlev, V. V.

citation count

• 6

complete list of authors

• Meng, Zhaokai||Hanson, Jessica A||Yakovlev, Vladislav V

editor list (cited editors)

• Rollins, A. M., Fraser, S. E., & Choma, M. A.

publication date

• May 2016

publisher

• SPIE, the international society for optics and photonics  Publisher

published in

• Proceedings of SPIE  Journal

keywords

• Brillouin Imaging
• Brillouin Spectroscopy
• Embryo Imaging
• Raman Spectroscopy
• Virtually Imaged Phased Array (vipa)
• Viscoelasticity-specific Imaging
• Zebrafish

Digital Object Identifier (DOI)

• 10.1117/12.2213978

International Standard Book Number (ISBN) 13

• 9781628419504

start page

• 97160l-97160l-6

volume

• 9716

URL

• http%3A%2F%2Fdx.doi.org%2F10.1117%2F12.2213978