Enhanced second-harmonic-generation detection of collagen by means of optical wavefront shaping Conference Paper uri icon


  • 2016 SPIE. Second-harmonic generation (SHG) has proven to be an effective method to both image and detect structural variations in fibrillar collagen. The ability to detect these differences is especially useful in studying diseases like cancer and fibrosis.1 SHG techniques have historically been limited by their ability to penetrate and image through strongly scattering tissues. Recently, optical wavefront shaping has enabled light to be focused through highly scattering media such as biological tissue.2-4 This technology also enables us to examine the dependence of second harmonic generation on the spatial phase of the pump laser. Here, we demonstrate that wavefront shaping can be used to enhance the generation of second harmonic light from collagen fibrils even when scattering is low or non-existent.

name of conference

  • Adaptive Optics and Wavefront Control for Biological Systems II

published proceedings


author list (cited authors)

  • Thompson, J. V., Throckmorton, G. A., Hokr, B. H., & Yakovlev, V. V.

citation count

  • 0

complete list of authors

  • Thompson, Jonathan V||Throckmorton, Graham A||Hokr, Brett H||Yakovlev, Vladislav V

editor list (cited editors)

  • Bifano, T. G., Kubby, J., & Gigan, S.

publication date

  • March 2016