Assessing the effect of a high-fat diet on rodents' adipose tissue using Brillouin and Raman spectroscopy Conference Paper uri icon


  • 2016 SPIE. The purpose of this study is to evaluate the effect of a high-lipid diet on elasticity of adipose tissue. We employed dual Raman/Brillouin microspectroscopy to analyze brown and white adipose tissues obtained from adult rats. The rats were divided into two groups, one of which received a high-fat feed, while the other served as a control. We hypothesized that the changes in the elasticity of adipose tissues between the two groups can be successfully assessed using Brillouin spectroscopy. We found that the brown adipose tissue possessed a lesser Brillouin shift than the white adipose within each group and that the elastic modulus of both adipose tissues increases in the high-fat diet group. The Raman spectra provided supplementary chemical information and indicated an increase in the lipid-to-protein ratio in the brown adipose, but not in the white adipose.

name of conference

  • Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis

published proceedings


author list (cited authors)

  • Troyanova-Wood, M., Gobbell, C., Meng, Z., & Yakovlev, V. V.

citation count

  • 2

complete list of authors

  • Troyanova-Wood, Maria||Gobbell, Cassidy||Meng, Zhaokai||Yakovlev, Vladislav V

editor list (cited editors)

  • Alfano, R. R., & Demos, S. G.

publication date

  • March 2016