Measurement of amino acid isotope enrichment by liquid chromatography mass spectroscopy after derivatization with 9-fluorenylmethylchloroformate. Academic Article

abstract

• An automated method is described to measure tracer-tracee-ratios (TTR) of plasma amino acids after their separation as 9-fluorenylmethylchloroformate derivatives. In a 45 min cycle, 5 microl plasma aliquots were derivatized, HPLC separated and subjected to electrospray ionization. By applying source collision induced dissociation, derivatives were dissociated at the peptide bond releasing the originating amino acids into the mass spectrometer. This approach enabled the determination of plasma amino acid TTRs with a standard deviation between 0.15 and 0.36%, which is sufficient to study the fate of infused tracers and their conversion products in an in vivo experiment in humans.

authors

• Deutz, Nicolaas

published proceedings

• J Chromatogr B Analyt Technol Biomed Life Sci

author list (cited authors)

• van Eijk, H., Suylen, D., Dejong, C., Luiking, Y. C., & Deutz, N.

citation count

• 22

complete list of authors

• van Eijk, Hans MH||Suylen, Dennis PL||Dejong, Cornelis HC||Luiking, Yvette C||Deutz, Nicolaas EP

publication date

• September 2007

publisher

• Elsevier  Publisher

published in

• Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences  Journal

keywords

• Aged
• Amino Acids
• Chromatography, High Pressure Liquid
• Fluorenes
• Humans
• Indicators And Reagents
• Male
• Middle Aged
• Reproducibility Of Results
• Spectrometry, Mass, Electrospray Ionization

PubMed Central ID

• 17561452

Digital Object Identifier (DOI)

• 10.1016/j.jchromb.2007.05.020

start page

• 48

end page

• 56

volume

• 856

issue

• 1-2

URL

• http://dx.doi.org/10.1016/j.jchromb.2007.05.020