High-speed label-free functional photoacoustic microscopy of mouse brain in action. Academic Article uri icon

abstract

  • We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

published proceedings

  • Nat Methods

altmetric score

  • 104.078

author list (cited authors)

  • Yao, J., Wang, L., Yang, J., Maslov, K. I., Wong, T., Li, L., ... Wang, L. V.

citation count

  • 458

complete list of authors

  • Yao, Junjie||Wang, Lidai||Yang, Joon-Mo||Maslov, Konstantin I||Wong, Terence TW||Li, Lei||Huang, Chih-Hsien||Zou, Jun||Wang, Lihong V

publication date

  • May 2015