High-speed label-free functional photoacoustic microscopy of mouse brain in action.

abstract

• We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

authors

• Zou, Jun

published proceedings

• Nat Methods

altmetric score

• 103.578

author list (cited authors)

• Yao, J., Wang, L., Yang, J., Maslov, K. I., Wong, T., Li, L., ... Wang, L. V.

citation count

• 499

complete list of authors

• Yao, Junjie||Wang, Lidai||Yang, Joon-Mo||Maslov, Konstantin I||Wong, Terence TW||Li, Lei||Huang, Chih-Hsien||Zou, Jun||Wang, Lihong V

publication date

• May 2015

publisher

• Springer Nature  Publisher

published in

• Nature Methods  Journal

keywords

• Animals
• Blood Flow Velocity
• Brain
• Electric Stimulation
• Female
• Mice
• Microscopy
• Photoacoustic Techniques
• Reproducibility Of Results

PubMed Central ID

• 25822799

Digital Object Identifier (DOI)

• 10.1038/nmeth.3336

start page

• 407

end page

• 410

volume

• 12

issue

• 5

URL

• http%3A%2F%2Fdx.doi.org%2F10.1038%2Fnmeth.3336