An improved RNA isolation method for plant tissues containing high levels of phenolic compounds or carbohydrates

abstract

• Difficulties extracting high-quality RNA from recalcitrant plant tissues are often due to high levels of phenolics, carbohydrates, or other compounds that bind and/or coprecipitate with RNA. We describe here a method using soluble polyvinyl pyrrolidone (PVP) and ethanol precipitation, which has been successful in several recalcitrant systems where other specialized RNA extraction methods failed to deliver suitable product. Using this method, RNA capable of reverse-transcription/PCR amplification and cDNA library construction was isolated from ripening grape berries, dry seeds of Albizia procera and radish, and leaf tissue of A. procera and Griffonia simplicifolia. This method is applicable to a variety of plant tissues.

authors

• Zhu Salzman, Keyan

published proceedings

• PLANT MOLECULAR BIOLOGY REPORTER

author list (cited authors)

• Salzman, R. A., Fujita, T., Zhu-Salzman, K., Hasegawa, P. M., & Bressan, R. A.

citation count

• 181

complete list of authors

• Salzman, RA||Fujita, T||Zhu-Salzman, K||Hasegawa, PM||Bressan, RA

publication date

• March 1999

publisher

• Springer Nature  Publisher

published in

• Plant Molecular Biology Reporter  Journal

keywords

• Albizia
• Griffonia
• Phenolics
• Recalcitrant Tissues
• Rna Extraction
• Vitis

Digital Object Identifier (DOI)

• 10.1023/A:1007520314478

start page

• 11

end page

• 17

volume

• 17

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1023%2Fa%3A1007520314478