Identification and confirmation of RAPD markers and andromonoecious associated with QTL for sucrose in muskmelon
Conference Paper
Overview
Research
Identity
Additional Document Info
Other
View All
Overview
abstract
Our objectives were to identify RAPD and andromonoecious (a) markers associated with QTL for sucrose using bulked segregant analysis in an F 2 population from the melon (Cucumis melo L.) cross of 'TAM Dulce' (high sucrose) TGR1551 (low sucrose) in a greenhouse experiment, and confirm the association of RAPD and a markers with sucrose QTL in an F 2 population from the different cross of 'Deltex' (high sucrose) TGR1551 in a field experiment. Continuous distributions for sucrose were observed in the F2 populations indicating quantitative inheritance for the trait. A significant positive correlation was found between sucrose and total soluble solids (TSS). Nine RAPD markers were detected to be significantly associated with QTL for sucrose in the F2 population of the 'TAM Dulce' TGR1551 cross in the greenhouse based on simple linear regression. Five unlinked markers associated with QTL were significant in a stepwise multiple regression analysis where the full model explained 39% of the total phenotypic variation for sucrose. Three to five of the nine RAPD markers were also observed to be significantly associated with QTL for glucose, fructose and TSS, respectively, suggesting that in this cross four sugar traits are controlled by the same QTL. Four RAPD markers were confirmed in the F 2 population of the 'Deltex' TGR1551 cross in the field to be consistently associated with sucrose QTL. A significant association of a with sucrose was consistently expressed in our populations under greenhouse and field environments.
