Identification of a protein that recognizes a distal negative regulatory element within the mouse mammary tumor virus long terminal repeat. Academic Article uri icon

abstract

  • The mouse mammary tumor virus (MMTV) long terminal repeat contains a distal negative regulatory element (dNRE) that selectively represses activity of the proviral promoter in the absence of steroid hormone receptor-mediated activation. A protein, termed MMTV NRE-binding protein 1 (MNBP-1), that recognizes long terminal repeat sequences between -433 and -418 was identified by gel electrophoresis mobility shift assays and methylation interference footprinting in nuclear extracts of HeLa and Ltk(-) cells. Mutations within the defined binding site affect dNRE-mediated promoter repression in vivo. MNBP-1 has an apparent molecular mass of approximately 100 kDa as determined by gel filtration chromatography.

published proceedings

  • Virology

author list (cited authors)

  • Kang, C. J., & Peterson, D. O.

complete list of authors

  • Kang, CJ||Peterson, DO

publication date

  • January 1, 1999 11:11 AM