Identification of a protein that recognizes a distal negative regulatory element within the mouse mammary tumor virus long terminal repeat. Academic Article

abstract

• The mouse mammary tumor virus (MMTV) long terminal repeat contains a distal negative regulatory element (dNRE) that selectively represses activity of the proviral promoter in the absence of steroid hormone receptor-mediated activation. A protein, termed MMTV NRE-binding protein 1 (MNBP-1), that recognizes long terminal repeat sequences between -433 and -418 was identified by gel electrophoresis mobility shift assays and methylation interference footprinting in nuclear extracts of HeLa and Ltk(-) cells. Mutations within the defined binding site affect dNRE-mediated promoter repression in vivo. MNBP-1 has an apparent molecular mass of approximately 100 kDa as determined by gel filtration chromatography.

authors

• Peterson, David

published proceedings

• Virology

author list (cited authors)

• Kang, C. J., & Peterson, D. O.

citation count

• 4

complete list of authors

• Kang, CJ||Peterson, DO

publication date

• November 1999

publisher

• Elsevier  Publisher

published in

• Virology  Journal

keywords

• Amino Acid Sequence
• Animals
• Base Sequence
• Cell Nucleus
• DNA-Binding Proteins
• Hela Cells
• Humans
• L Cells
• Mammary Tumor Virus, Mouse
• Mice
• Molecular Sequence Data
• Proviruses
• Regulatory Sequences, Nucleic Acid
• Sequence Alignment
• Sequence Homology, Nucleic Acid
• Terminal Repeat Sequences

Digital Object Identifier (DOI)

• 10.1006/viro.1999.9969

start page

• 211

end page

• 219

volume

• 264

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1006%2Fviro.1999.9969