Fecal microbiome and metabolites differ between breast and formula-fed human infants Conference Paper uri icon


  • Herein, an integrated analysis of the fecal microbiota, microbiome and metabolites of 3monthold breast (BF; n=6) or formulafed (FF; n=6) infants was performed. Microbial composition and functional microbiome were accessed by 16S rDNA amplicon and shotgun metagenomic pyrosequencing, respectively. Fecal metabolites were measured by gas chromatography/mass spectrometry. Distancebased redundancy analysis distinguished the microbiota of BF from FF. BF infants harbored more Bacteroidetes, while FF had more Firmicutes and Verrucomicrobia (p<0.05). The microbiomes of BF and FF infants contained higher proportions of protein/amino acid vs. carbohydrate metabolism genes, respectively. Partial leastsquares discriminant analysis discriminated fecal metabolomic profiles of BF vs. FF. The major metabolites enriched in BF infants were 15methylhexadecanoic acid, galactitol and maltose. The metabolites associated with FF were betaalanine, dodecanoic acid, glycolic acid, decanoic acid and tyramine. Thus, early nutrition impacts the composition of the fecal microbiota, as well as the complement of microbial genes and metabolic products. (BMS Freedom to Discover Award).Grant Funding Source: BMS Freedom to Discover Award

published proceedings


author list (cited authors)

  • Wang, M., Li, M., Chapkin, R. S., Ivanov, I., & Donovan, S. M.

citation count

  • 2

complete list of authors

  • Wang, Mei||Li, Min||Chapkin, Robert S||Ivanov, Ivan||Donovan, Sharon M

publication date

  • April 2013