Case Study of Processing and Insemination Techniques: Attempts to Improve Fertility of an Aged Stallion with Dilute Semen of Poor Quality Academic Article uri icon

abstract

  • The objective of this case study was to investigate whether semen centrifugation and low-dose insemination techniques would improve fertility of an aged subfertile Quarter Horse stallion with low sperm concentration, motility, and morphology in ejaculates. Forty-five mares were bred by one of five treatments (n = 9 per group) using the entire ejaculate as follows: (1) Group Body: body insemination with ejaculate diluted 1:1 in TAMU extender; (2) Group Body-Cent: body insemination after centrifugation and re-suspension of sperm pellet to 1 mL in TAMU extender; (3) Group Horn-Cent: deep horn insemination after centrifugation and re-suspension of sperm pellet to 1 mL in TAMU extender; (4) Group Cent-Hys: hysteroscopic insemination onto the uterotubal papilla after centrifugation and re-suspension of sperm pellet to 200 L in Kenney-Modified Tyrode's extender; and (5) Group Dens-Hys: hysteroscopic insemination onto the uterotubal papilla after discontinuous density gradient centrifugation and re-suspension of the sperm pellet in 200-L Kenney-Modified Tyrode's extender. Pregnancy rates did not differ among treatment groups (P =77). Semen centrifugation for low dose insemination did not appear to improve fertility of this subfertile stallion, despite use of entire ejaculates for each individual insemination dose. 2012 Elsevier Inc.

published proceedings

  • Journal of Equine Veterinary Science

author list (cited authors)

  • Blanchard, T. L., Brinsko, S. P., Love, C. C., Vest, D. D., Berezowski, C. B., Wendt, K. M., Stich, K., & Varner, D. D.

complete list of authors

  • Blanchard, Terry L||Brinsko, Steven P||Love, Charles C||Vest, Dickey D||Berezowski, Christopher B||Wendt, Konnie M||Stich, Kelly||Varner, Dickson D

publication date

  • January 1, 2012 11:11 AM