Unraveling the molecular basis of temperature-dependent genetic regulation in Penicillium marneffei.
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Penicillium marneffei is an opportunistic fungal pathogen endemic in Southeast Asia, causing lethal systemic infections in immunocompromised patients. P. marneffei grows in a mycelial form at the ambient temperature of 25C and transitions to a yeast form at 37C. The ability to alternate between the mycelial and yeast forms at different temperatures, namely, thermal dimorphism, has long been considered critical for the pathogenicity of P. marneffei, yet the underlying genetic mechanisms remain elusive. Here we employed high-throughput sequencing to unravel global transcriptional profiles of P. marneffei PM1 grown at 25 and 37C. Among 11,000 protein-coding genes, 1,447 were overexpressed and 1,414 were underexpressed at 37C. Counterintuitively, heat-responsive genes, predicted in P. marneffei through sequence comparison, did not tend to be overexpressed at 37C. These results suggest that P. marneffei may take a distinct strategy of genetic regulation at the elevated temperature; the current knowledge concerning fungal heat response, based on studies of model fungal organisms, may not be applicable to P. marneffei. Our results further showed that the tandem repeat sequences (TRSs) are overrepresented in coding regions of P. marneffei genes, and TRS-containing genes tend to be overexpressed at 37C. Furthermore, genomic sequences and expression data were integrated to characterize gene clusters, multigene families, and species-specific genes of P. marneffei. In sum, we present an integrated analysis and a comprehensive resource toward a better understanding of temperature-dependent genetic regulation in P. marneffei.
author list (cited authors)
Yang, E., Wang, G., Woo, P., Lau, S., Chow, W., Chong, K., ... Cai, J. J.
complete list of authors
Yang, Ence||Wang, Gang||Woo, Patrick CY||Lau, Susanna KP||Chow, Wang-Ngai||Chong, Ken TK||Tse, Herman||Kao, Richard YT||Chan, Che-Man||Che, Xiaoyan||Yuen, Kwok-Yung||Cai, James J