Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin.
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Milo disease in sorghum is caused by isolates of the soil-borne fungus Periconia circinata that produce PC-toxin. Susceptibility to milo disease is conditioned by a single, semi-dominant gene, termed Pc. The susceptible allele (Pc) converts to a resistant form (pc) spontaneously at a gametic frequency of 10(-3) to 10(-4). A high-density genetic map was constructed around the Pc locus using DNA markers, allowing the Pc gene to be delimited to a 0.9 cM region on the short arm of sorghum chromosome 9. Physically, the Pc-region was covered by a single BAC clone. Sequence analysis of this BAC revealed twelve gene candidates. Several of the predicted genes in the region are homologous to disease resistance loci, including one NBS-LRR resistance gene analogue that is present in multiple tandem copies. Analysis of pc isolines derived from Pc/Pc sorghum suggests that one or more members of this NBS-LRR gene family are the Pc genes that condition susceptibility.
author list (cited authors)
Nagy, E. D., Lee, T., Ramakrishna, W., Xu, Z., Klein, P. E., SanMiguel, P., ... Bennetzen, J. L.
complete list of authors
Nagy, Ervin D||Lee, Tso-Ching||Ramakrishna, Wusirika||Xu, Zijun||Klein, Patricia E||SanMiguel, Phillip||Cheng, Chiu-Ping||Li, Jingling||Devos, Katrien M||Schertz, Keith||Dunkle, Larry||Bennetzen, Jeffrey L