Substrates, Wounding, and Growth Regulator Concentrations Alter Adventitious Rooting of Baldcypress Cuttings Academic Article uri icon


  • In previous studies, baldcypress [Taxodium distichum (L.) Rich.] clones were selected for tolerance to high pH soils, drought and salt exposures, and ornamental characteristics. The objective of the current research was to determine the treatment combinations that yielded optimum root quantity (percentage) and rooted cutting quality (root number, length, dry mass, and shoot dry mass) on vegetative cuttings for a representative clone. Cuttings were treated with factorial combinations of one of four potassium salt of indole-3-butyric acid (K-IBA) concentrations [0, 5,000, 10,000, 15,000 mgL1 (0, 20.72, 41.44, 62.16 mm, respectively)], wounded or not wounded (1-cm long basal incision), and rooted in one of three substrates (100% perlite, 100% peatmoss, or 50% perlite:50% peatmoss). Data indicated a tradeoff between potential rooting quantity and root quality measurements in response to different substrates. Although rooting percentages were affected by substrates only at P 0.10 (53% in 100% perlite versus 36% in 100% peatmoss), there were highly significant (P 0.0001) differences in rooted cutting potential among substrates as measured by the percentage of cuttings with basal callus. Cuttings placed in 100% perlite callused at 85%, whereas cuttings placed in 100% peatmoss callused at 53%. The 100% peatmoss treatment, however, yielded cuttings with significantly greater root quality for all measurements, except root number per cutting. Wounding cuttings proved to have deleterious effects on root quality measurements. Total root length was 14.5 cm for non-wounded cuttings and 10.8 cm for wounded cuttings. Increasing K-IBA concentrations did not significantly (P 0.05) affect rooting or callus percentages but did significantly affect root dry mass, total root length, and average root length per cutting. Total root length increased from 10.8 cm at 0 mgL1 K-IBA to 16 cm at 15,000 mgL1 K-IBA. Mean root number per cutting increased from 1.6 with wounded cuttings planted in 100% peatmoss to 3.1 with non-wounded cuttings planted in 100% perlite. Results suggested that high-quality softwood baldcypress cuttings should not be wounded, should be treated with 15,000 mgL1 K-IBA, and grown in a substrate with intermediate water-holding capacity to achieve an acceptable balance between rooting percentage and rooted cutting quality objectives.

published proceedings


author list (cited authors)

  • King, A. R., Arnold, M. A., Welsh, D. F., & Watson, W. T.

citation count

  • 2

complete list of authors

  • King, Andrew R||Arnold, Michael A||Welsh, Douglas F||Watson, W Todd

publication date

  • October 2011