Rat subcutaneous adipose tissue glutamine release using arteriovenous differences
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abstract
The net release of glutamine and glycerol by rat subcutaneous adipose tissue was determined using aortic arterial and epigastric venous plasma concentration differences. Male Sprague Dawley rats in the fed state (3588g (meanSEM), n=8), 48h starved state (2905g n=7) or 48h starved/72h refed state (3254g n=8), were anesthetized with chloral hydrate (450 mg/kg i.p.). The aorta and epigastric vein were exposed and blood was collected simultaneously. Plasma concentrations of glutamine were determined using HPLC after OP A derivatization, and glycerol using a microfluorometric procedure. A net release of glutamine was seen in all states, with an arteriovenous (AV) difference of-90+35, -11939, and -11526 nM in the fed, starved, and refed states. These AV difference values are similar to the arterial whole blood - tissue (AT) difference of -12218n.M derived from microdialysis studies in fed rats (Kowalski and Watford, Am. J. Physiol. 266:E151, 1994). There was a net release of glycerol with an AV difference of -12228, -30158, and -11317 |iM in the fed, starved, and refed states, with the value in starved rats significantly higher than fed or refed rats. The glycerol AV difference value for the fed and refed rats is similar to an AT difference of -12142 M from microdialysis studies in fed rats. These AV difference results show a net release of glutamine and glycerol by rat subcutaneous adipose tissue. These data demonstrate that rat subcutaneous adipose tissue metabolism can be assessed using an AV djtrerence technique.
