Effect of grain sorghum protein digestibility on starch gelatinization and enzymatic conversion to glucose
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The objective of this study is to select the best high digestible grain sorghum (HDGS) lines that breeders have developed for low energy input liquefaction, saccharification and fermentation for bio-ethanol production. The parental lines and offspring generated by crosses of two HD lines (P850029 and P851171) with three wild type (WT) sorghum lines (B.T635, R.T436 and 96GCPOB124) were used in this study. After the identification of recombinant inbred lines (RILs) as high, medium and normal protein digestible grain sorghum using the protease turbidity assay, the flour pasting properties of the sorghum samples were examined using rapid visco-analyzer (RVA). Results showed that the HDGS lines with modified endosperm matrices has reduced temperature and duration needed to solubilize the grain starch for hydrolytic enzyme access and conversion to fermentable sugars.