DELIMITATION OF THE LE2DAV COMPLEMENTARY LETHALITY SYSTEM OF GOSSYPIUM TO INTRACELLULAR INTERACTION
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We investigated the basis of hybrid necrosis and lethality in cotton (Gossypium spp.) caused by interactions of Le2dav with Le1 and Le2 with regard to the possible roles of intracellular and intercellular genetic interactions in eliciting the hybrid necrosis. Seedlings chimeric for sectors bearing the complementary hybrid lethality factors Le2dav vs. Le1Le2 were derived by crossing facultatively apomictic (semigamy) SeSeLe1Le1Le2Le2 ovule parents with nonapomictic pollen parents of the genotype sesele1le1Le2davLe2dav. We used virescent mutant (v1) and glandless mutant (gl2gl3) markers to facilitate identification of parental origins of haploids and sectors of chimeric haploids. F1 hybrid progenies resulting from normal reproduction became necrotic and perished before or shortly after germination. F1 hybrid sectors of chimeric progeny also became necrotic. Necrosis, however, was relegated to F1 sectors and did not diffuse. Furthermore, boundaries between paternal and maternal sectors of chimeras were free of the necrotic reaction. Our results indicate that events underlying the hybrid lethality reaction are mediated via intracellular interactions and that intercellular diffusates are not involved. 1989 The American Genetic Association.
