Optical axial scanning in confocal microscopy using an electrically tunable lens.

abstract

• This paper presents the use and characterization of an electrically focus tunable lens to perform axial scanning in a confocal microscope. Lateral and axial resolution are characterized over a >250 m axial scan range. Confocal microscopy using optical axial scanning is demonstrated in epithelial tissue and compared to traditional stage scanning. By enabling rapid axial scanning, minimizing motion artifacts, and reducing mechanical complexity, this technique has potential to enhance in vivo three-dimensional imaging in confocal endomicroscopy.

authors

• Cheng, Yi-Shing
• Wright, John

published proceedings

• Biomed Opt Express

author list (cited authors)

• Jabbour, J. M., Malik, B. H., Olsovsky, C., Cuenca, R., Cheng, S., Jo, J. A., ... Maitland, K. C.

citation count

• 105

complete list of authors

• Jabbour, Joey M||Malik, Bilal H||Olsovsky, Cory||Cuenca, Rodrigo||Cheng, Shuna||Jo, Javier A||Cheng, Yi-Shing Lisa||Wright, John M||Maitland, Kristen C

publication date

• February 2014

publisher

• Optica Publishing Group  Publisher

published in

• Biomedical Optics Express  Journal

keywords

• Confocal Microscopy
• Imaging Systems
• Medical And Biological Imaging
• Medical Optics Instrumentation
• Three-dimensional Microscopy

PubMed Central ID

• 24575357

Digital Object Identifier (DOI)

• 10.1364/BOE.5.000645

start page

• 645

end page

• 652

volume

• 5

issue

• 2

URL

• http%3A%2F%2Fdx.doi.org%2F10.1364%2Fboe.5.000645