Volumetric structured illumination microscopy enabled by a tunable-focus lens. Academic Article uri icon

abstract

  • We present a mechanical-scan-free method for volumetric imaging of biological tissue. The optical sectioning is provided by structured illumination, and the depth of the imaging plane is varied using an electrically tunable-focus lens. We characterize and evaluate the ability of this axial-scanning mechanism in structured illumination microscopy and demonstrate its ability to perform subcellular resolution imaging in oral mucosa ex vivo. The proposed mechanism can potentially convert any wide-field microscope to a 3D-imaging platform without the need for mechanical scanning of imaging optics and/or sample.

author list (cited authors)

  • Hinsdale, T., Malik, B. H., Olsovsky, C., Jo, J. A., & Maitland, K. C.

citation count

  • 14

publication date

  • October 2015