Volumetric structured illumination microscopy enabled by a tunable-focus lens. Academic Article

abstract

• We present a mechanical-scan-free method for volumetric imaging of biological tissue. The optical sectioning is provided by structured illumination, and the depth of the imaging plane is varied using an electrically tunable-focus lens. We characterize and evaluate the ability of this axial-scanning mechanism in structured illumination microscopy and demonstrate its ability to perform subcellular resolution imaging in oral mucosa ex vivo. The proposed mechanism can potentially convert any wide-field microscope to a 3D-imaging platform without the need for mechanical scanning of imaging optics and/or sample.

authors

• Maitland, Kristen

published proceedings

• Opt Lett

author list (cited authors)

• Hinsdale, T., Malik, B. H., Olsovsky, C., Jo, J. A., & Maitland, K. C.

citation count

• 18

complete list of authors

• Hinsdale, Taylor||Malik, Bilal H||Olsovsky, Cory||Jo, Javier A||Maitland, Kristen C

publication date

• November 2015

publisher

• Optica Publishing Group  Publisher

published in

• Optics Letters  Journal

keywords

• Animals
• Cattle
• Equipment Design
• Equipment Failure Analysis
• Image Enhancement
• Imaging, Three-Dimensional
• In Vitro Techniques
• Lenses
• Lighting
• Microscopy
• Mouth Mucosa
• Reproducibility Of Results
• Sensitivity And Specificity

PubMed Central ID

• 26512489

Digital Object Identifier (DOI)

• 10.1364/OL.40.004943

start page

• 4943

end page

• 4946

volume

• 40

issue

• 21