Antibody-mediated bacterial clearance from the lower respiratory tract of mice requires complement component C3. Academic Article uri icon


  • To assess the contribution of complement to respiratory immunity in the context of a natural bacterial infection, we used mice genetically deficient in complement components and the murine pathogen Bordetella bronchiseptica. Complement component C3 was not required for the control of bacterial infection or for the generation of infection-induced protective immunity. However, C3-deficient (C3(-/-)) mice were severely defective, compared to wild type, in vaccine-induced protective immunity. Adoptively transferred immune serum from convalescent wild-type or C3(-/-) animals rapidly cleared B. bronchiseptica from the lungs of wild-type mice but did not affect its growth in C3(-/-) mice, indicating that the defect is not in the generation of protective immunity, but in its function. Immune serum was effective in C5-deficient mice but had little effect in the lungs of mice lacking either Fcgamma receptors (FcgammaR) or CR3, suggesting bacterial clearance is not via direct complement-mediated lysis. Together, these data indicate that complement is required for antibody-mediated clearance of Bordetella and suggest the mechanism involves C3 opsonization of bacteria for phagocytosis that is both CR3- and FcgammaR-dependent.

published proceedings

  • Eur J Immunol

altmetric score

  • 3

author list (cited authors)

  • Pishko, E. J., Kirimanjeswara, G. S., Pilione, M. R., Gopinathan, L., Kennett, M. J., & Harvill, E. T

citation count

  • 28

complete list of authors

  • Pishko, Elizabeth J||Kirimanjeswara, Girish S||Pilione, Mylisa R||Gopinathan, Lakshmi||Kennett, Mary J||Harvill, Eric T

publication date

  • January 2004