Effect of cyclosporine on human sperm motility in vitro. Academic Article

abstract

• Cyclosporine affects motility and viability of human sperm when incubated together in vitro. Sperm motility was almost reduced to nil following 10 min of incubation with cyclosporine at a concentration of 1 mg/mL. However, 200 micrograms/mL of the drug has no effect on motility and viability when tested for up to 60 min under standard laboratory conditions. Cyclosporine effect on sperm was both dose and time dependent. Sperm sensitivity and susceptibility to cyclosporine even to lower doses increased significantly following withdrawal of bovine serum albumin from the incubating medium. Compared to untreated controls, lactate dehydrogenase was estimated higher by more than 2 to 4 times in the sperm-free incubating media, suggesting an altered membrane porosity in the affected spermatozoa.

authors

• Chaki, Sankar

published proceedings

• Arch Androl

author list (cited authors)

• Misro, M. M., Chaki, S. P., Srinivas, M., & Chaube, S. K.

citation count

• 12

complete list of authors

• Misro, MM||Chaki, SP||Srinivas, M||Chaube, SK

publication date

• January 1999

publisher

• Taylor & Francis  Publisher

published in

• Systems Biology in Reproductive Medicine  Journal

keywords

• Cyclosporine
• Humans
• Immunosuppressive Agents
• In Vitro Techniques
• Male
• Sperm Motility

PubMed Central ID

• 10624505

Digital Object Identifier (DOI)

• 10.1080/014850199262526

start page

• 215

end page

• 220

volume

• 43

issue

• 3

URL

• http%3A%2F%2Fdx.doi.org%2F10.1080%2F014850199262526