Characterization of 10 polymorphic loci in the Baird's pocket gopher (Geomys breviceps) and cross-amplification in other gopher species
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Ten polymorphic microsatellite loci were isolated and characterized for the Baird's pocket gopher, Geomys breviceps. Each locus was screened in 40 individuals from 4 different populations found throughout College Station, Texas. The number of alleles per locus ranged from 2 to 16 and observed heterozygosity ranged from 0.100 to 1.000. Deviation from Hardy-Weinberg and linkage disequilibrium was also analyzed. These 10 microsatellite primers, as well as 4 previously described primers developed for Thomomys, also were tested for amplification in additional species of pocket gophers. These microsatellite loci will provide useful tools in population genetic studies of G. breviceps, as well as other pocket gopher species. 2011 Springer Science+Business Media B.V.