Eight novel polymorphic microsatellites in the hispid pocket mouse (Chaetodipus hispidus) and cross-amplification in other Perognathinae species (Rodentia: Heteromyidae)
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abstract
Twenty-eight nuclear microsatellites were isolated for the hispid pocket mouse, Chaetodipus hispidus, and characterized in 33 individuals from four genetically distinct groups throughout the geographic range of the species. Dinucleotide and trinucleotide microsatellite repeat motifs were screened, and of the 28 primer pairs created, eight amplified and were polymorphic. After correction for multiple tests, no loci deviated significantly from Hardy-Weinberg expectations. Working primers also were tested in other closely related species found within the subfamily Perognathinae. The microsatellite markers characterized in this study will be beneficial towards future population genetic research within the hispid pocket mouse and other Perognathinae rodents. 2012 Springer Science+Business Media B.V.
