Differential activation of NF-kappa B in colonic mucosa of DSS-challenged rats consuming fermentable fiber sources Conference Paper uri icon


  • NFB activation is associated with colon inflammation. We compared dietary fiber effects on SCFA, microbiota and NFB activation in a colitis model. Rats received diets containing corn oil and: 6% cellulose (CELL); or 2% CELL with 4% of PROMITOR soluble corn fiber (SC), PROMITOR resistant starch (RS), or SC+RS (MIX) for 2 wk before DSS (3%, 2, 14 d separation) (n=20/diet) or control treatment (no DSS, n=10/diet). Tissues were collected 10 d after DSS for NFB activity, injury and inflammation scores. Feces were analyzed for SCFA (M/g) and microbiota (qPCR 16S rRNA). DSS elevated SCFA (P<0.03), except for CELL. Butyrate concentration was not affected by DSS, yet % butyrate decreased in DSS rats (P<0.05 for CELL and RS). Enhancement of NFB activity by DSS was 25, 50, 61, and 32% for the CELL, SC, RS, and MIX diets, significant for all but CELL. Injury scores were not affected by CELL, but were >75% higher with SC and MIX. Changes in microbiota occurred as rats transitioned from a pelleted diet to experimental diets. Bifidobacteria numbers were elevated with the RS diet. These data suggest in rats consuming n6 PUFA, that SCFA production from altered microbiota is correlated with NFB activity in this colitis model. It is important to elucidate the underlying mechanisms to appreciate how dietary fiber may influence colon inflammation. Support: Tate & Lyle Health & Nutritional Sciences.

published proceedings


author list (cited authors)

  • Turner, N. D., Taddeo, S. S., Callaway, E. S., Fan, Y. Y., Davidson, L. A., Thomas, L. N., ... Lupton, J. R.

citation count

  • 0

complete list of authors

  • Turner, Nancy D||Taddeo, Stella S||Callaway, Evelyn S||Fan, Yang Y||Davidson, Laurie A||Thomas, Laura N||Ferguson, Carly M||Sturino, Joseph M||Chapkin, Robert S||Lupton, Joanne R

publication date

  • April 2010