Construction of a Cotton VIGS Library for Functional Genomics Study Chapter uri icon

abstract

  • To facilitate cotton functional genomics studies and identify cotton genes mediating biotic and abiotic stress responses, we report here a detailed protocol of construction of a VIGS library from diploid cotton Gossypium raimondii. Sequencing of the representative colonies revealed that the library covers a significant percentage of unique cotton genes. Based on the number of colonies in the primary library, it is estimated that this VIGS library is about 50× coverage of predicted cotton protein-coding genes. As a proof-of-concept experiment, we further describe a protocol to identify the genetic determinants involved in cotton drought tolerance using this VIGS library. The cotton VIGS library established here provides a unique opportunity to identify essential cotton genes in various stress responses and developmental growth regulation.

author list (cited authors)

  • Li, M., Li, F., & He, P.

citation count

  • 5

complete list of authors

  • Li, Maoying||Li, Fangjun||He, Ping

Book Title

  • Plant Gene Silencing

publication date

  • January 2015