Characterization of cholinesterase activity from different tissues of Nile tilapia (Oreochromis niloticus). Academic Article

abstract

• Cholinesterases (ChE) from brain, muscle and liver in Nile tilapia (Oreochromis niloticus) were characterized using three substrates: acetylthiocholine iodide, propionylthiocholine iodide, and butyrylthiocholine iodide. Eserine was used as a total ChE inhibitor; BW284c51 and iso-OMPA were used as selective inhibitors for acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), respectively. The results indicate that AChE is the enzyme present in brain, whereas in both liver and muscle, the presence of atypical ChEs are suggested. These findings indicate that characterization of ChE is necessary prior to use in monitoring programs.

authors

• Gold Bouchot, Gerardo

published proceedings

• Mar Environ Res

author list (cited authors)

• Rodrguez-Fuentes, G., & Gold-Bouchot, G.

citation count

• 61

complete list of authors

• Rodríguez-Fuentes, Gabriela||Gold-Bouchot, Gerardo

publication date

• August 2004

publisher

• Elsevier  Publisher

published in

• Marine Environmental Research  Journal

keywords

• Acetylthiocholine
• Analysis Of Variance
• Animals
• Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(n,n-dimethyl-n-2-propenyl-), Dibromide
• Brain
• Butyrylthiocholine
• Cholinesterase Inhibitors
• Cholinesterases
• Cichlids
• Environmental Monitoring
• Kinetics
• Liver
• Male
• Muscle, Skeletal
• Physostigmine
• Regression Analysis
• Tetraisopropylpyrophosphamide
• Thiocholine

PubMed Central ID

• 15178074

Digital Object Identifier (DOI)

• 10.1016/j.marenvres.2004.03.037

start page

• 505

end page

• 509

volume

• 58

issue

• 2-5

URL

• http://dx.doi.org/10.1016/j.marenvres.2004.03.037