Formation and decay of prelumirhodopsin at room temperatures. Academic Article uri icon

abstract

  • We have excited detergent-solubilized bovine rhodopsin at room temperature with 530-nm light pulses from a mode locked laser, and have observed the appearance and decay of a transient species that absorbs more strongly at 560 nm than does ground-state rhodopsin. Our data show that the absorbing intermediate appears in a time that is at least as short as the experimental resolution (about 6 psec) and decays with a life time of about 30 nsec. The extremely fast risetime supports the hypothesis that prelumirhodopsin is the product of the primary photoprocess.

published proceedings

  • Proc Natl Acad Sci U S A

author list (cited authors)

  • Busch, G. E., Applebury, M. L., Lamola, A. A., & Rentzepis, P. M.

citation count

  • 233

complete list of authors

  • Busch, GE||Applebury, ML||Lamola, AA||Rentzepis, PM

publication date

  • October 1972