In vitro oxidation of essential amino acids by jejunal mucosal cells of growing pigs uri icon

abstract

  • This study was conducted to test the hypothesis that porcine jejunal mucosal cells can degrade all essential amino acids (EAA). Jejunal mucosal cells (primarily enterocytes) were isolated from 50-day-old healthy pigs and incubated at 37C for 45min in Krebs buffer containing plasma concentrations of amino acids and one of the following l-[1-14C]- or l-[U-14C]-amino acids plus unlabeled tracers at 0.5, 2, or 5mM: histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine. There was active transamination of leucine, isoleucine and valine in the cells (1.35-2.5nmol/mg protein per 45min at 2mM), with most (67-71%) of their -ketoacids released into the medium. In contrast, catabolism of methionine and phenylalanine was negligible (0.04-0.05nmol/mg protein per 45min at 2mM) and that of other EAA was completely absent in these cells. These results indicate that intestinal mucosal cells are a site for the substantial degradation of branched-chain amino acids but not other EAA in pigs. 2007 Elsevier B.V. All rights reserved.

published proceedings

  • Livestock Science

author list (cited authors)

  • Chen, L., Yin, Y., Jobgen, W. S., Jobgen, S. C., Knabe, D. A., Hu, W., & Wu, G.

citation count

  • 51

complete list of authors

  • Chen, Lixiang||Yin, Yu-Long||Jobgen, Wenjuan S||Jobgen, Scott C||Knabe, Darrell A||Hu, Wei-Xin||Wu, Guoyao

publication date

  • May 2007