In vitro oxidation of essential amino acids by jejunal mucosal cells of growing pigs
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This study was conducted to test the hypothesis that porcine jejunal mucosal cells can degrade all essential amino acids (EAA). Jejunal mucosal cells (primarily enterocytes) were isolated from 50-day-old healthy pigs and incubated at 37C for 45min in Krebs buffer containing plasma concentrations of amino acids and one of the following l-[1-14C]- or l-[U-14C]-amino acids plus unlabeled tracers at 0.5, 2, or 5mM: histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine. There was active transamination of leucine, isoleucine and valine in the cells (1.35-2.5nmol/mg protein per 45min at 2mM), with most (67-71%) of their -ketoacids released into the medium. In contrast, catabolism of methionine and phenylalanine was negligible (0.04-0.05nmol/mg protein per 45min at 2mM) and that of other EAA was completely absent in these cells. These results indicate that intestinal mucosal cells are a site for the substantial degradation of branched-chain amino acids but not other EAA in pigs. 2007 Elsevier B.V. All rights reserved.
author list (cited authors)
Chen, L., Yin, Y., Jobgen, W. S., Jobgen, S. C., Knabe, D. A., Hu, W., & Wu, G.
complete list of authors
Chen, Lixiang||Yin, Yu-Long||Jobgen, Wenjuan S||Jobgen, Scott C||Knabe, Darrell A||Hu, Wei-Xin||Wu, Guoyao