In situ hybridization mapping and restriction fragment length polymorphism analysis of the porcine albumin (ALB) and transferrin (TF) genes. Academic Article

abstract

• In situ hybridization analyses were conducted on porcine metaphase chromosomes using porcine liver albumin (ALB) and transferrin (TF) cDNA probes. The ALB gene was assigned to the q12 band of chromosome 8 and the TF gene to the q31 band of chromosome 13. For the latter, a statistically significant secondary peak was observed on the 6p15 band. However, the TF probe predominantly hybridized to the 13q31 band, indicating that this band is the most likely site of the TF gene. Since the TF gene belongs to linkage group V, this linkage group can now be assigned to chromosome 13. The TF and ALB probes were also used for restriction fragment length polymorphism (RFLP) analysis. A screening of 10 unrelated animals revealed TaqI RFLPs for both ALB and TF. Family studies indicated that the ALB and TF polymorphisms were controlled by three and two alleles, respectively.

authors

• Andersson, Leif

published proceedings

• Anim Genet

author list (cited authors)

• Chowdhary, B. P., Johansson, M., Chaudhary, R., Ellegren, H., Gu, F., Andersson, L., & Gustavsson, I.

citation count

• 26

complete list of authors

• Chowdhary, BP||Johansson, M||Chaudhary, R||Ellegren, H||Gu, F||Andersson, L||Gustavsson, I

publication date

• April 1993

publisher

• Wiley  Publisher

published in

• Animal Genetics  Journal

keywords

• Alleles
• Animals
• Autoradiography
• Chromosome Banding
• Chromosome Mapping
• DNA Probes
• Genes, Dominant
• Genetic Linkage
• In Situ Hybridization
• Metaphase
• Polymorphism, Restriction Fragment Length
• Serum Albumin
• Swine
• Transferrin

PubMed Central ID

• 8101068

Digital Object Identifier (DOI)

• 10.1111/j.1365-2052.1993.tb00245.x

start page

• 85

end page

• 90

volume

• 24

issue

• 2

URL

• http://dx.doi.org/10.1111/j.1365-2052.1993.tb00245.x