Integrated plastic microfluidic device for ssDNA separation Academic Article uri icon


  • An integrated microfluidic device with on-chip photodiode is fabricated for single strand DNA (ssDNA) separation and detection. The microfluidic component is fabricated by parylene thin-wall technology. In order to achieve oxygen free environment for acrylamide gel polymerization in microfluidic channel, alumina inner coating is introduced to decrease parylene thin film permeability. On-chip high sensitivity photodiode successfully detected ssDNA fluorescence at the end of separation. Results show alumina is a good oxygen barrier which secures excellent cross-linking of the sieving matrix. Single base pair resolution for ssDNA separation in this device has been achieved. 2007 Elsevier B.V. All rights reserved.

published proceedings


altmetric score

  • 3

author list (cited authors)

  • Zheng, J., Webster, J. R., Mastrangelo, C. H., Ugaz, V. M., Burns, M. A., & Burke, D. T.

citation count

  • 10

complete list of authors

  • Zheng, Jun||Webster, James R||Mastrangelo, Carlos H||Ugaz, Victor M||Burns, Mark A||Burke, David T

publication date

  • July 2007