Integrated plastic microfluidic device for ssDNA separation Academic Article

abstract

• An integrated microfluidic device with on-chip photodiode is fabricated for single strand DNA (ssDNA) separation and detection. The microfluidic component is fabricated by parylene thin-wall technology. In order to achieve oxygen free environment for acrylamide gel polymerization in microfluidic channel, alumina inner coating is introduced to decrease parylene thin film permeability. On-chip high sensitivity photodiode successfully detected ssDNA fluorescence at the end of separation. Results show alumina is a good oxygen barrier which secures excellent cross-linking of the sieving matrix. Single base pair resolution for ssDNA separation in this device has been achieved. 2007 Elsevier B.V. All rights reserved.

authors

• Ugaz, Victor

published proceedings

• SENSORS AND ACTUATORS B-CHEMICAL

altmetric score

• 3

author list (cited authors)

• Zheng, J., Webster, J. R., Mastrangelo, C. H., Ugaz, V. M., Burns, M. A., & Burke, D. T.

citation count

• 10

complete list of authors

• Zheng, Jun||Webster, James R||Mastrangelo, Carlos H||Ugaz, Victor M||Burns, Mark A||Burke, David T

publication date

• July 2007

publisher

• Elsevier  Publisher

published in

• Sensors and Actuators, B: Chemical  Journal

keywords

• Alumina
• Microfluidic Device
• Parylene
• Photodiode
• SsDNA

Digital Object Identifier (DOI)

• 10.1016/j.snb.2007.02.036

start page

• 343

end page

• 351

volume

• 125

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.snb.2007.02.036