Enhanced resolution in microchip dna electrophoresis by tailoring hydrogel nanostructure to exploit entropic trapping
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Macromolecules embedded within a nanoporous matrix experience entropic trapping (ET) when their dimensions become close to the average pore size - a regime distinguished by emergence of anomalous transport behavior. But these phenomena have not been widely explored in hydrogel matrices because it has not been clear how they are linked to the underlying complex heterogeneous nanopore morphology. Here we introduce a theoretical model that establishes this connection, and describe microchip DNA electrophoresis experiments guided by these insights that demonstrate how ET effects can be exploited to yield a surprising trend of increasing resolving power with DNA size (the opposite of what is conventionally observed).
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
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