Lab-on-a-drone deployment of nucleic acid-based diagnostics
Additional Document Info
15CBMS-0001. We describe an ultra-portable bioanalysis platform for rapid nucleic acid-based diagnostics using consumer-class quadcopter drones. Our approach exploits the ability to isothermally actuate the polymerase chain reaction (PCR) with a single heater, enabling the system to be operated using standard 5 V USB sources that power mobile devices. Time-resolved fluorescence detection and quantification are achieved using a smartphone camera and integrated image analysis app. Functionality is demonstrated in the context of infectious disease diagnostics (Ebola, Staphylococcus aureus, and Dengue). These advancements make it possible to overcome existing price, performance, portability, and ruggedization.