FISH mapping of the IGF2 gene in horse and donkey-detection of homoeology with HSA11. Academic Article

abstract

• Three genomic subclones derived from a phage clone containing the equine IGF2 gene were used to FISH map the gene on horse (ECA) and donkey (EAS) metaphase chromosomes. The gene mapped on ECA 12q13 band and is the first locus mapped to this horse chromosome. In donkey the gene mapped very terminal on the long arm of one small submetacentric chromosome that shows almost identical DAPI-banding pattern with ECA12. This is the first locus mapped in donkey genome. Cross species chromosome painting of equine metaphase chromosomes with human Chromosome (Chr) 11-specific probe showed homoeology of this human chromosome with ECA12 and ECA7. The novel ECA12 comparative painting results are thus in accordance with the localization of the equine IGF2 gene. Comparison of the hitherto known physical locations of IGF2 in different species, viz. human, cattle, sheep, horse, and donkey, shows that this gene tends to maintain a terminal location on the chromosome arm.

authors

• Raudsepp, Terje

published proceedings

• Mamm Genome

author list (cited authors)

• Raudsepp, T., Otte, K., Rozell, B., & Chowdhary, B. P.

citation count

• 18

complete list of authors

• Raudsepp, T||Otte, K||Rozell, B||Chowdhary, BP

publication date

• January 1997

publisher

• Springer Nature  Publisher

published in

• MAMMALIAN GENOME  Journal

keywords

• Animals
• Blotting, Southern
• Chromosome Mapping
• Chromosomes
• Equidae
• Horses
• In Situ Hybridization, Fluorescence
• Insulin-Like Growth Factor II
• Karyotyping
• Metaphase

Digital Object Identifier (DOI)

• 10.1007/s003359900505

start page

• 569

end page

• 572

volume

• 8

issue

• 8

URL

• http%3A%2F%2Fdx.doi.org%2F10.1007%2Fs003359900505