The 450-band resolution G- and R-banded standard karyotype of the donkey (Equus asinus, 2n = 62). Academic Article uri icon

abstract

  • Donkey chromosomes were earlier characterized separately by C-, G- and R-banding techniques. However, direct comparisons between G- and R-banding patterns have still not been carried out in this species. The present study reports this comparison at the 450-band level by using replication G- and R-banding patterns. Two sets of synchronized lymphocyte cultures were set up to obtain early (GBA+CBA-banding) and late (RBA-banding) BrdU incorporation. Slides were stained with acridine orange and observed under a fluorescence microscope. Reverse GBA+CBA- and RBA-banded karyotypes at the 450-band level were constructed. To verify G- and R-banding patterns in some acrocentric chromosomes, sequential GBA+CBA/Ag-NORs and RBA/Ag-NORs were also performed. The results of CBA-banding patterns obtained in 12 animals from 2 breeds showed a pronounced polymorphism of heterochromatin, especially in EAS1q-prox. Ideogrammatic representations of G- and R-banded karyotypes were constructed using only one common G- and R-banding nomenclature. In the present study both G- and R-banding patterns and relative ideograms are presented as standard karyotype for this species at the 450-band level.

published proceedings

  • Cytogenet Genome Res

author list (cited authors)

  • Di Meo, G. P., Perucatti, A., Peretti, V., Incarnato, D., Ciotola, F., Liotta, L., ... Iannuzzi, L.

citation count

  • 7

complete list of authors

  • Di Meo, GP||Perucatti, A||Peretti, V||Incarnato, D||Ciotola, F||Liotta, L||Raudsepp, T||Di Berardino, D||Chowdhary, B||Iannuzzi, L

publication date

  • January 2009