In vitro maturation of equine oocytes obtained from different age groups of sexually mature mares. Academic Article

abstract

• Oocytes were harvested from mare ovaries obtained at slaughter and were divided into 3 groups based on the age of the donor. The age groups consisted of young (2 to 7 yr), middle-aged (8 to 14 yr) and aged (>or=15 yr) mares. There were no differences between age groups in the proportions of follicles available for examination or the proportions of normal, abnormal or total oocytes collected. After 24 h of culture, the overall maturation rate to the second metaphase (MII) was 52.7%. Maturation rates for oocytes obtained from young and middle-aged mares were similar, but oocytes from aged mares were only approximately 25% as likely to reach MII and they were 3 times more likely to remain at metaphase I. Twelve oocytes had chromosome spreads suitable for counting; 6 were haploid, 2 were hyperhaploid and 4 were hypohaploid. Insufficient numbers of readable spreads precluded comparisons of chromosome complements between age groups.

authors

• Brinsko, Steven

published proceedings

• Theriogenology

author list (cited authors)

• Brinsko, S. P., Ball, B. A., & Ellington, J. E.

citation count

• 24

complete list of authors

• Brinsko, SP||Ball, BA||Ellington, JE

publication date

• January 1995

publisher

• Elsevier  Publisher

published in

• THERIOGENOLOGY  Journal

keywords

• Age
• Equine
• In Vitro
• Maturation
• Oocytes

Digital Object Identifier (DOI)

• 10.1016/0093-691x(95)00218-w

start page

• 461

end page

• 469

volume

• 44

issue

• 4

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2F0093-691x%2895%2900218-w