Relationship between stallion sperm motility and viability as detected by two fluorescence staining techniques using flow cytometry. Academic Article

abstract

• Relationships between sperm motility parameters and viability were evaluated using two fluorescent staining techniques in fresh extended semen (fresh and after 24 h storage at 5 degrees C) that had various concentrations of dead sperm added to simulate different levels of viable and nonviable sperm. Both protocols incorporated SYBR-14 and propidium iodide (PI) while the second protocol added the mitochondrial probe JC-1. The relationship between total sperm motility and percent viable sperm was high between staining protocols (r = 0.98). Time (0 h versus 24 h, P<0.0001) and treatment (0, 10, 25, 50, and 75% nonviable sperm, P<0.0001) affected percent total sperm motility and percent viable sperm for both staining protocols. Actual percent viable sperm for each time and treatment did not differ from expected values.

authors

• Brinsko, Steven
• Love, Charles
• Varner, Dickson

published proceedings

• Theriogenology

author list (cited authors)

• Love, C. C., Thompson, J. A., Brinsko, S. P., Rigby, S. L., Blanchard, T. L., Lowry, V. K., & Varner, D. D.

citation count

• 60

complete list of authors

• Love, CC||Thompson, JA||Brinsko, SP||Rigby, SL||Blanchard, TL||Lowry, VK||Varner, DD

publication date

• January 2003

publisher

• Elsevier  Publisher

published in

• THERIOGENOLOGY  Journal

keywords

• Animals
• Benzimidazoles
• Carbocyanines
• Cell Survival
• Flow Cytometry
• Fluorescent Dyes
• Horses
• Male
• Organic Chemicals
• Propidium
• Sperm Motility
• Spermatozoa
• Staining And Labeling

PubMed Central ID

• 12935852

Digital Object Identifier (DOI)

• 10.1016/s0093-691x(03)00122-5

start page

• 1127

end page

• 1138

volume

• 60

issue

• 6

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fs0093-691x%2803%2900122-5