Relationship between stallion sperm motility and viability as detected by two fluorescence staining techniques using flow cytometry. Academic Article uri icon


  • Relationships between sperm motility parameters and viability were evaluated using two fluorescent staining techniques in fresh extended semen (fresh and after 24 h storage at 5 degrees C) that had various concentrations of dead sperm added to simulate different levels of viable and nonviable sperm. Both protocols incorporated SYBR-14 and propidium iodide (PI) while the second protocol added the mitochondrial probe JC-1. The relationship between total sperm motility and percent viable sperm was high between staining protocols (r = 0.98). Time (0 h versus 24 h, P<0.0001) and treatment (0, 10, 25, 50, and 75% nonviable sperm, P<0.0001) affected percent total sperm motility and percent viable sperm for both staining protocols. Actual percent viable sperm for each time and treatment did not differ from expected values.

published proceedings

  • Theriogenology

author list (cited authors)

  • Love, C. C., Thompson, J. A., Brinsko, S. P., Rigby, S. L., Blanchard, T. L., Lowry, V. K., & Varner, D. D.

citation count

  • 59

complete list of authors

  • Love, CC||Thompson, JA||Brinsko, SP||Rigby, SL||Blanchard, TL||Lowry, VK||Varner, DD

publication date

  • October 2003