Assessment of using laponite cross-linked poly(ethylene oxide) for controlled cell adhesion and mineralization.

abstract

• The in vitro cytocompatibility of silicate (Laponite clay) cross-linked poly(ethylene oxide) (PEO) nanocomposite films using MC3T3-E1 mouse preosteoblast cells was investigated while cell adhesion, spreading, proliferation and mineralization were assessed as a function of film composition. By combining the advantageous characteristics of PEO polymer (hydrophilic, prevents protein and cell adhesion) with those of a synthetic and layered silicate (charged, degradable and potentially bioactive) some of the physical and chemical properties of the resulting polymer nanocomposites could be controlled. Hydration, dissolution and mechanical properties were examined and related to cell adhesion. Overall, this feasibility study demonstrates the ability of using model Laponite cross-linked PEO nanocomposites to create bioactive scaffolds.

authors

• Gaharwar, Akhilesh

published proceedings

• Acta Biomater

author list (cited authors)

• Gaharwar, A. K., Schexnailder, P. J., Kline, B. P., & Schmidt, G.

complete list of authors

• Gaharwar, Akhilesh K||Schexnailder, Patrick J||Kline, Benjamin P||Schmidt, Gudrun

publisher

• Elsevier  Publisher

published in

• Acta Biomaterialia  Journal

keywords

• Alkaline Phosphatase
• Animals
• Calcification, Physiologic
• Cell Adhesion
• Cell Death
• Cell Movement
• Cell Proliferation
• Cell Survival
• Cross-Linking Reagents
• Materials Testing
• Mechanical Phenomena
• Mice
• Nanocomposites
• Nanoparticles
• Osteoblasts
• Polyethylene Glycols
• Silicates
• Surface Properties

PubMed Central ID

• 20854941

Digital Object Identifier (DOI)

• 10.1016/j.actbio.2010.09.015

start page

• 568

end page

• 577

volume

• 7

issue

• 2