RNA sequencing for transcript 5'-end mapping in mycobacteria. Academic Article

abstract

• Next-generation sequencing technologies facilitate the analysis of multiple important properties of the transcriptome in addition to gene expression levels. Here we describe a method for mapping RNA 5' ends in Mycobacterium tuberculosis, which allows the determination of transcriptional start sites (TSSs), comparative analysis of promoter usage under different conditions, and mapping of endoribonucleolytic processing sites. We describe in detail the procedures for constructing RNA sequencing libraries appropriate for RNA 5' end mapping using an Illumina sequencing platform. We also outline the major steps of data analysis.

authors

• Ioerger, Thomas

published proceedings

• Methods Mol Biol

altmetric score

• 1.25

author list (cited authors)

• Shell, S. S., Chase, M. R., Ioerger, T. R., & Fortune, S. M.

citation count

• 9

complete list of authors

• Shell, Scarlet S||Chase, Michael R||Ioerger, Thomas R||Fortune, Sarah M

publication date

• January 2015

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Gene Expression
• Gene Library
• High-Throughput Nucleotide Sequencing
• Mycobacterium
• RNA, Messenger
• Sequence Analysis, RNA
• Transcription, Genetic

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-2450-9_3

start page

• 31

end page

• 45

volume

• 1285

URL

• http%3A%2F%2Fdx.doi.org%2F10.1007%2F978-1-4939-2450-9_3

user-defined tag

• 3 Good Health and Well-Being