RNA sequencing for transcript 5'-end mapping in mycobacteria. Academic Article uri icon

abstract

  • Next-generation sequencing technologies facilitate the analysis of multiple important properties of the transcriptome in addition to gene expression levels. Here we describe a method for mapping RNA 5' ends in Mycobacterium tuberculosis, which allows the determination of transcriptional start sites (TSSs), comparative analysis of promoter usage under different conditions, and mapping of endoribonucleolytic processing sites. We describe in detail the procedures for constructing RNA sequencing libraries appropriate for RNA 5' end mapping using an Illumina sequencing platform. We also outline the major steps of data analysis.

published proceedings

  • Methods Mol Biol

altmetric score

  • 1.25

author list (cited authors)

  • Shell, S. S., Chase, M. R., Ioerger, T. R., & Fortune, S. M.

citation count

  • 8

complete list of authors

  • Shell, Scarlet S||Chase, Michael R||Ioerger, Thomas R||Fortune, Sarah M

publication date

  • February 2015