Relationship of bursal anti-steroidogenic peptide (BASP) and histone H1. Academic Article uri icon

abstract

  • Previous in vitro research from our laboratory has demonstrated the existence of a protein purified from the chicken bursa of Fabricius, with potent antisteroidogenic and antiproliferative action on granulose cells and lymphocytes, respectively called Bursal anti-steroidogenic peptide (BASP). This protein is heat-labile, basic, and amino- and carboxy-terminus blocked. In highly purified form, the protein presents as a doublet on SDS-PAGE electrophoresis with an apparent MW of approximately 29 and approximately 32 kDa. Recently, Nanoflow Q-TOF Mass Spectrometry amino acid sequencing allowed determination of a convincing partial amino acid sequence, strongly suggesting a probable relationship of BASP with histone H1. Bursal cDNA expression library screening, using an antibody produced against BASP, also identified a clone with a sequence matching histone H1. Presently, we have demonstrated that SDS-PAGE electrophoresis of highly purified and bioactive BASP, and commercially-available calf thymus derived histone H1, produced similar doublets at approximately the same apparent MW, and that the electrophoretic profile of these 2 preparations were strikingly similar following 2 dimensional gel electrophoresis. The BASP doublet produced on SDS-PAGE was recognized by a commercially available monoclonal antibody recognizing a highly conserved region of histone H1. Furthermore, calf thymus histone H1 was found to suppress mitogen-stimulated chicken B-cell proliferation in a concentration-related manner, similar to the action of BASP. These data indicate that BASP shares substantial structural homology with, and may be identical to, histone H1.

published proceedings

  • Life Sci

author list (cited authors)

  • Garca-Espinosa, G., Moore, R. W., Berghman, L. R., & Hargis, B. M.
  • García-Espinosa, G., Moore, R. W., Berghman, L. R., & Hargis, B. M
  • García-Espinosa, G., Moore, R. W., Berghman, L. R., & Hargis, B. M.

citation count

  • 3

complete list of authors

  • García-Espinosa, Gary||Moore, Randy W||Berghman, Luc R||Hargis, Billy M

publication date

  • November 2002