Optimal conditions and specificity of interaction of a distinct class of nonhistone chromosomal proteins with DNA. Academic Article uri icon

abstract

  • A subclass of nonhistone chromatin proteins with high DNA affinity has been isolated from rat liver. The interaction of the isolated proteins with DNA in vitro was characterized utilizing a nitrocellulose filter binding technique. The temperature, time, concentration, ionic strength, and pH dependence were characterized. Optimal interaction was observed at 0.19 M naCl, pH 7.5 with a protein to DNA ratio of 13 (w/w). Equilibrium and kinetic competition experiments indicated that these proteins interact optimally with A-T rich and single-stranded DNA. The data also suggest that these proteins might affect the helixcoil transiton of DNA.

published proceedings

  • Biochemistry

author list (cited authors)

  • Thomas, T. L., & Patel, G. L.

citation count

  • 39

complete list of authors

  • Thomas, TL||Patel, GL

publication date

  • April 1976