Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites. Academic Article

abstract

• Interspersed repetitive sequences were isolated from sea urchin DNA by renaturing to low Cot followed by treatment with nuclease SI. Synthetic Eco RI sites were ligated onto the repetitive sequence elements, which were then inserted at the Eco RI site of plasmid RSF2124 and cloned. The repetitive sequences can be excised from the plasmid with Eco RI for further study.

authors

• Thomas, Terry

published proceedings

• Science

author list (cited authors)

• Scheller, R. H., Thomas, T. L., Lee, A. S., Klein, W. H., Niles, W. D., Britten, R. J., & Davidson, E. H.

citation count

• 55

complete list of authors

• Scheller, RH||Thomas, TL||Lee, AS||Klein, WH||Niles, WD||Britten, RJ||Davidson, EH

publication date

• April 1977

publisher

• American Association for the Advancement of Science (AAAS)  Publisher

published in

• Science  Journal

keywords

• Base Sequence
• DNA Restriction Enzymes
• DNA, Recombinant
• Molecular Weight
• Nucleic Acid Renaturation
• Oligodeoxyribonucleotides
• Polynucleotide Ligases
• Sea Urchins

PubMed Central ID

• 847467

Digital Object Identifier (DOI)

• 10.1126/science.847467

start page

• 197

end page

• 200

volume

• 196

issue

• 4286

URL

• http%3A%2F%2Fdx.doi.org%2F10.1126%2Fscience.847467