Investigation of enzyme formulation on pretreated switchgrass.
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This work studied the benefits of adding different enzyme cocktails (cellulase, xylanase, -glucosidase) to pretreated switchgrass. Pretreatment methods included ammonia fiber expansion (AFEX), dilute-acid (DA), liquid hot water (LHW), lime, lime+ball-milling, soaking in aqueous ammonia (SAA), and sulfur dioxide (SO(2)). The compositions of the pretreated materials were analyzed and showed a strong correlation between initial xylan composition and the benefits of xylanase addition. Adding xylanase dramatically improved xylan yields for SAA (+8.4%) and AFEX (+6.3%), and showed negligible improvement (0-2%) for the pretreatments with low xylan content (dilute-acid, SO(2)). Xylanase addition also improved overall yields with lime+ball-milling and SO(2) achieving the highest overall yields from pretreated biomass (98.3% and 93.2%, respectively). Lime+ball-milling obtained an enzymatic yield of 92.3kg of sugar digested/kg of protein loaded.
Falls, M., Shi, J., Ebrik, M. A., Redmond, T., Yang, B., Wyman, C. E., ... Holtzapple, M. T.
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Falls, Matthew||Shi, Jian||Ebrik, Mirvat A||Redmond, Tim||Yang, Bin||Wyman, Charles E||Garlock, Rebecca||Balan, Venkatesh||Dale, Bruce E||Pallapolu, V Ramesh||Lee, YY||Kim, Youngmi||Mosier, Nathan S||Ladisch, Michael R||Hames, Bonnie||Thomas, Steve||Donohoe, Bryon S||Vinzant, Todd B||Elander, Richard T||Warner, Ryan E||Sierra-Ramirez, Rocio||Holtzapple, Mark T