Competitive binding assay for glucose based on glycodendrimer-fluorophore conjugates. Academic Article uri icon


  • A new fluorescent glucose assay has been created using Alexa Fluor 647-labeled concanavalin A (Con A) and a fourth-generation PAMAM Alexa Fluor 594-labeled glycodendrimer. This assay has been shown to have a large response to glucose within the biological range and to be capable of functioning within a polymer hydrogel. In this paper, the glucose response is shown to be a single fluorophore-based quenching reaction. Data showing that the sensor is fully reversible and specific through competitive binding between the dendrimer and glucose with Con A are presented. Overall, the assay is shown to have potential over the traditional dextran-based assay because it has a larger dynamic response to physiological glucose concentrations, incorporates longer wavelength dyes that improve signal penetration through dermal tissue, and provides an internal reference in the form of a nonreactive fluorescent label.

published proceedings

  • Anal Chem

altmetric score

  • 3

author list (cited authors)

  • Ibey, B. L., Beier, H. T., Rounds, R. M., Cot, G. L., Yadavalli, V. K., & Pishko, M. V.

citation count

  • 46

complete list of authors

  • Ibey, Bennett L||Beier, Hope T||Rounds, Rebecca M||Coté, Gerard L||Yadavalli, Vamsi K||Pishko, Michael V

publication date

  • November 2005