Rational design of a bisphenol A aptamer selective surface-enhanced Raman scattering nanoprobe. Academic Article uri icon


  • Surface-enhanced Raman scattering (SERS) optical nanoprobes offer a number of advantages for ultrasensitive analyte detection. These functionalized colloidal nanoparticles are a multifunctional assay component. providing a platform for conjugation to spectral tags, stabilizing polymers, and biorecognition elements such as aptamers or antibodies. We demonstrate the design and characterization of a SERS-active nanoprobe and investigate the nanoparticles' biorecognition capabilities for use in a competitive binding assay. Specifically, the nanoprobe is designed for the quantification of bisphenol A (BPA) levels in the blood after human exposure to the toxin in food and beverage plastic packaging. The nanoprobes demonstrated specific affinity to a BPA aptamer with a dissociation constant Kd of 54 nM, and provided a dose-dependent SERS spectra with a limit of detection of 3 nM. Our conjugation approach shows the versatility of colloidal nanoparticles in assay development, acting as detectable spectral tagging elements and biologically active ligands concurrently.

published proceedings

  • Anal Chem

altmetric score

  • 0.5

author list (cited authors)

  • Marks, H. L., Pishko, M. V., Jackson, G. W., & Cot, G. L.

citation count

  • 69

complete list of authors

  • Marks, Haley L||Pishko, Michael V||Jackson, George W||Coté, Gerard L

publication date

  • December 2014