A low dilution rate microchemostat array with programmable cell population control
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Chemostat is a bioreactor in which microorganisms can be cultured at steady state by controlling the rate of medium inflow and waste outflow. Microfluidic chemostat arrays can provide higher throughput with significantly smaller reagent consumption while still providing accurate chemostatic condition control, all at significantly lower costs compared to conventional chemostats. Here we present a microchemostat array that can provide wide ranges of dilution rates from as low as 0.01/h to 1/h in a 250 nl volume chemostat. The compact size of the device housing 8 arrays on a 4 by 6 cm footprint allows high-throughput microbial studies. Each chemostat unit also has integrated electrodes for on-line pH sensing and programmable population control mechanism. Copyright (2011) by the Chemical and Biological Microsystems Society.
